Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Gastroprotective Effects of DAS-77 (a Phytomedicine) in Ulcer Models in Rats

Abidemi J Akindele¹ , Flora R Aigbe¹, Johnson A Olowe², Babajide O Oduntan¹, Olufunmilayo O Adeyemi¹

¹Department of Pharmacology; ²Department of Physiology, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, PMB 12003, Lagos, Nigeria.

For correspondence:- Abidemi Akindele Email: ajakindele@cmul.edu.ng Tel:+2348062359726

Received: 20 February 2012 Accepted: 27 August 2012 Published: 18 October 2012

Citation: Akindele AJ, Aigbe FR, Olowe JA, Oduntan BO, Adeyemi OO. Gastroprotective Effects of DAS-77 (a Phytomedicine) in Ulcer Models in Rats. Trop J Pharm Res 2012; 11(5):783-791 doi: 10.4314/tjpr.v11i5.12

© 2012 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: DAS-77 is a phytomedicine that contains the dried bark of Mangifera indica and root of Carica papaya. This study investigated the antiulcer effects of DAS-77 in rats.

Methods: DAS-77 was administered orally twice daily for five consecutive days at doses of 50 - 400 mg/kg. Ulcer was induced in rats with ethanol, indomethacin, pylorus ligation (PL) and cold restraint stress (CRS). Ulcer scores were recorded based on examination of excised stomachs. Estimations of gastric content volume, pH and titratable acidity in the PL model and determination of the levels of antioxidants and malondialdehyde (MDA) in gastric tissues in the CRS model were also done.

Results: In all the models, DAS-77 produced significant dose-dependent reductions in ulcer score. Peak effects were produced at the dose of 400 mg/kg with ulcer inhibition values of 98.57, 76.23, 99.28 and 96.70 % compared to 100.00, 93.79, 98.92 and 96.79 % for misoprostol/cimetidine, respectively, for the ethanol, indomethacin, PL and CRS models. In the PL model, DAS-77 caused a significant increase in pH of gastric content but a reduction in volume and titratable acidity. At doses of 50 and 100 mg/kg in the CRS model, DAS-77 significantly increased the level of reduced glutathione (GSH) and diminished MDA.

Conclusion: The results obtained in this study suggest that DAS-77 possesses gastroprotective activity possibly due to reduced gastric secretion and acidity, and antioxidant activity.

Keywords: DAS-77, Phytomedicine, Mangifera indica, Carica papaya, Gastroprotective effects, Ulcer